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flow cytometry sony id7000 spectral cell analyzer  (Sony)

 
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    Structured Review

    Sony flow cytometry sony id7000 spectral cell analyzer
    Flow Cytometry Sony Id7000 Spectral Cell Analyzer, supplied by Sony, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometry+sony+id7000+spectral+cell+analyzer/sh800+cell+sorter/10__1016_slash_j__snb__2025__138310-102-5-7
    Average 90 stars, based on 1 article reviews
    flow cytometry sony id7000 spectral cell analyzer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Enhanced lung delivery of an immunostimulatory duplex RNA augments the antitumor activity by reshaping systemic cytokine pharmacodynamics
    Article Snippet: Following staining, cells were fixed using fixation buffer (BioLegend) and washed before loading into a 96-well plate U-bottom (Eppendorf). .. Stained cells were analyzed using flow cytometry with a Sony iD7000 spectral flow cytometer and the data were analyzed using FlowJo software. .. Animal care and experimentation Wild-type (WT) female C57BL/6 mice (6-10 weeks old) were obtained from Charles River Laboratories.

    Article Title: ERα blockade in dendritic cells enhances antigen cross-presentation and induces antitumor CD8 + T cell immunity.
    Article Snippet: We used the CellTraceTM CFSE Cell Proliferation Kit (C34554; Thermo) for CFSE staining according to the manufacturer’s protocol. .. For analysis of mouse DC isotype, 1×106 DCs were blocked with TruStain FcXTM PLUS (anti-mouse CD16/32) antibodies at 4 °C for 10 min, then stained with PE or APC-anti-mouse CD11c antibody, FITCanti-mouse CD80 antibody, PE-Cy7-anti-mouse CD86 antibody, APC or PE anti-mouse H2Kb bound to SIINFEKL antibody and FITC anti-mouse I-A/I-E antibody for 30 min. For analysis of human DC isotype, 1×106 DCs were stained with BV421 anti-human HLA-DR antibody, PECF594 or BV605-anti-human CD86 antibody, BV510-anti-human CD40 antibody and PE-Cy7anti-human CD274 antibody for 30 min. Stained cells were analyzed by ID7000 (SONY) BD LSRFortessa or BD FACSymphony A1 flow cytometer and analyzed using FlowJo version 10 software. ..

    Flow Cytometry:

    Article Title: Enhanced lung delivery of an immunostimulatory duplex RNA augments the antitumor activity by reshaping systemic cytokine pharmacodynamics
    Article Snippet: Following staining, cells were fixed using fixation buffer (BioLegend) and washed before loading into a 96-well plate U-bottom (Eppendorf). .. Stained cells were analyzed using flow cytometry with a Sony iD7000 spectral flow cytometer and the data were analyzed using FlowJo software. .. Animal care and experimentation Wild-type (WT) female C57BL/6 mice (6-10 weeks old) were obtained from Charles River Laboratories.

    Article Title: Allogeneic CRISPR-Engineered CAR-T Cells Drive Potent Antitumor Activity in Solid Tumors
    Article Snippet: The bioluminescence signal flux was analyzed using the Living Image Software (Revvity) as photons per second per square centimeter per steradian (photons/s/cm 2 /sr). .. Flow cytometry was conducted using the Sony ID7000, and the data were analyzed with FlowJo software. .. For the mouse model studies, the Xenogen IVIS Lumina (PerkinElmer) was utilized, and the Living Image Software (Revvity) was employed to assess the bioluminescence signal flux.

    Article Title: Targeting Lactate-Driven Stromal Autophagy via MCT1 Disrupts the Immunosuppressive Niche and Sensitizes Pancreatic Cancer to PD-1 Blockade.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reati ncubated with various flow cytometry antibodies (see KRT) at oom temperature for 1 h. Finally, after washing, the cells were nalyzed by flow cytometer (Sony ID7000). .. .12 Confocal Microscopy lentiviral vector containing the green fluorescent protein GFP)-red fluorescent protein (RFP)-LC3B plasmid was contructed by GeneChem (Shanghai, China).

    Article Title: A TIM3+ CD27+ Tissue-Resident CD8+ T Cell Population Unique to Chronically Rejected Lungs
    Article Snippet: Purpose: T cells drive CLAD pathogenesis, especially cytotoxic CD8 T cells.. We have identified a CD8 T cell population defined by expression of TIM3 and CD27 that is prominent in CLAD lungs while absent from donor lungs (Fig A-B, D) and hypothesize that this represents a lung tissue-resident population that promotes injury of the transplanted lung.. Methods: Using scRNAseq analysis and spectral flow cytometry (using Sony ID7000) we compared CLAD lungs and peripheral blood mononuclear cells (PBMC) obtained from re-transplant or autopsy with control lungs obtained from excess donor tissue. scRNAseq was performed on lymphocyte-enriched cells using the 10X Genomics platform.

    Article Title: Chronic Lung Allograft Dysfunction (CLAD) is Characterized by Reduced Secretoglobin SCGB3A2
    Article Snippet: Correlation of Endogenous Club Cell Secretory Protein (CCSP) and Fibrosis in Chronic Lung Allograft Dysfunction (CLAD) Z. Carter, A. Burman, B. Renaud-Picard, G. Berra, D. Hwang, A.L.. Pilon, A.E.. Gelman, S. Juvet, and T. Martinu.

    Article Title: ERα blockade in dendritic cells enhances antigen cross-presentation and induces antitumor CD8 + T cell immunity.
    Article Snippet: We used the CellTraceTM CFSE Cell Proliferation Kit (C34554; Thermo) for CFSE staining according to the manufacturer’s protocol. .. For analysis of mouse DC isotype, 1×106 DCs were blocked with TruStain FcXTM PLUS (anti-mouse CD16/32) antibodies at 4 °C for 10 min, then stained with PE or APC-anti-mouse CD11c antibody, FITCanti-mouse CD80 antibody, PE-Cy7-anti-mouse CD86 antibody, APC or PE anti-mouse H2Kb bound to SIINFEKL antibody and FITC anti-mouse I-A/I-E antibody for 30 min. For analysis of human DC isotype, 1×106 DCs were stained with BV421 anti-human HLA-DR antibody, PECF594 or BV605-anti-human CD86 antibody, BV510-anti-human CD40 antibody and PE-Cy7anti-human CD274 antibody for 30 min. Stained cells were analyzed by ID7000 (SONY) BD LSRFortessa or BD FACSymphony A1 flow cytometer and analyzed using FlowJo version 10 software. ..

    Software:

    Article Title: Enhanced lung delivery of an immunostimulatory duplex RNA augments the antitumor activity by reshaping systemic cytokine pharmacodynamics
    Article Snippet: Following staining, cells were fixed using fixation buffer (BioLegend) and washed before loading into a 96-well plate U-bottom (Eppendorf). .. Stained cells were analyzed using flow cytometry with a Sony iD7000 spectral flow cytometer and the data were analyzed using FlowJo software. .. Animal care and experimentation Wild-type (WT) female C57BL/6 mice (6-10 weeks old) were obtained from Charles River Laboratories.

    Article Title: Allogeneic CRISPR-Engineered CAR-T Cells Drive Potent Antitumor Activity in Solid Tumors
    Article Snippet: The bioluminescence signal flux was analyzed using the Living Image Software (Revvity) as photons per second per square centimeter per steradian (photons/s/cm 2 /sr). .. Flow cytometry was conducted using the Sony ID7000, and the data were analyzed with FlowJo software. .. For the mouse model studies, the Xenogen IVIS Lumina (PerkinElmer) was utilized, and the Living Image Software (Revvity) was employed to assess the bioluminescence signal flux.

    Article Title: ERα blockade in dendritic cells enhances antigen cross-presentation and induces antitumor CD8 + T cell immunity.
    Article Snippet: We used the CellTraceTM CFSE Cell Proliferation Kit (C34554; Thermo) for CFSE staining according to the manufacturer’s protocol. .. For analysis of mouse DC isotype, 1×106 DCs were blocked with TruStain FcXTM PLUS (anti-mouse CD16/32) antibodies at 4 °C for 10 min, then stained with PE or APC-anti-mouse CD11c antibody, FITCanti-mouse CD80 antibody, PE-Cy7-anti-mouse CD86 antibody, APC or PE anti-mouse H2Kb bound to SIINFEKL antibody and FITC anti-mouse I-A/I-E antibody for 30 min. For analysis of human DC isotype, 1×106 DCs were stained with BV421 anti-human HLA-DR antibody, PECF594 or BV605-anti-human CD86 antibody, BV510-anti-human CD40 antibody and PE-Cy7anti-human CD274 antibody for 30 min. Stained cells were analyzed by ID7000 (SONY) BD LSRFortessa or BD FACSymphony A1 flow cytometer and analyzed using FlowJo version 10 software. ..

    other:

    Article Title: Attenuated FTO induces necroptosis of alveolar epithelium via the m 6 A/CYP1B1/ROS/MLKL axis to promote the aggravation of pulmonary emphysema.
    Article Snippet: Finally, immunophenotyping and population quantification were conducted using flow cytometry (Sony ID7000).

    Article Title: PEBP4 alleviates muscle wasting in lung cancer cachexia via KEAP1-NRF2-mediated redox homeostasis.
    Article Snippet: Cells were incubated in serum-free medium containing DHE for 1 h at 37 °C, then collected, washed, and analyzed by flow cytometry (ID7000, Sony, Japan; 10,000 events/sample).

    Control:

    Article Title: A TIM3+ CD27+ Tissue-Resident CD8+ T Cell Population Unique to Chronically Rejected Lungs
    Article Snippet: Purpose: T cells drive CLAD pathogenesis, especially cytotoxic CD8 T cells.. We have identified a CD8 T cell population defined by expression of TIM3 and CD27 that is prominent in CLAD lungs while absent from donor lungs (Fig A-B, D) and hypothesize that this represents a lung tissue-resident population that promotes injury of the transplanted lung.. Methods: Using scRNAseq analysis and spectral flow cytometry (using Sony ID7000) we compared CLAD lungs and peripheral blood mononuclear cells (PBMC) obtained from re-transplant or autopsy with control lungs obtained from excess donor tissue. scRNAseq was performed on lymphocyte-enriched cells using the 10X Genomics platform.

    Article Title: Chronic Lung Allograft Dysfunction (CLAD) is Characterized by Reduced Secretoglobin SCGB3A2
    Article Snippet: Correlation of Endogenous Club Cell Secretory Protein (CCSP) and Fibrosis in Chronic Lung Allograft Dysfunction (CLAD) Z. Carter, A. Burman, B. Renaud-Picard, G. Berra, D. Hwang, A.L.. Pilon, A.E.. Gelman, S. Juvet, and T. Martinu.



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